Anti-TLX1 Polyclonal Antibody for WB, ELISA - P31314

Core Product Specifications and Parameters

Biological Background: TLX1 Function and Localization

• TLX1, also known as Hox-11, is a homeobox transcription factor critical for spleen organogenesis.
• It binds specifically to the DNA sequence 5'-GGCGGTAAGTGG-3' to regulate target gene expression.
• As a member of the homeobox gene family, TLX1 contains a homeodomain that mediates DNA binding.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• TLX1 is classified as a proto-oncogene; chromosomal rearrangements involving the gene are associated with T-cell acute lymphoblastic leukemia.
• The gene undergoes alternative splicing, and the protein is subject to acetylation, which may modulate its activity.

Experimental Guidance and Technical Tips

• The immunogen, spanning amino acids 1-190 of human TLX1, corresponds to a region highly conserved in human, mouse, and rat, supporting the antibody's reported cross-reactivity.
• For Western blotting, the predicted molecular weight is 34 kDa; it is advisable to validate the antibody in relevant tissue lysates (e.g., spleen) and include appropriate loading controls.
• For ELISA applications, consider using this polyclonal antibody as either a capture or detection reagent, with optimization of coating conditions and concentrations.
• As a rabbit polyclonal antibody, it recognizes multiple epitopes, which may enhance sensitivity but also requires careful blocking to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering a wide range of validated antibodies and ancillary reagents to support diagnostic development. For research on spleen development and leukemia involving TLX1, we provide optimized polyclonal antibodies like this one, along with bulk supply options and customized sourcing for rare targets, helping you move seamlessly from concept to clinic.