Anti-TLR9 Polyclonal Antibody for WB, IHC-P, ELISA - Q9NR96

Core Product Specifications and Parameters

Biological Background: Toll-like receptor 9 Function and Localization

• TLR9 is a key component of innate and adaptive immunity, recognizing unmethylated CpG dinucleotides in pathogens. Related references: PMID:14716310
• Acts via MYD88 and TRAF6 to activate NF-kappa-B, leading to cytokine secretion and inflammatory response. Related references: PMID:11564765, PMID:17932028, PMID:40980882
• Also signals through ADCY7 to produce cyclic di-AMP, activating the NLRP3 inflammasome (By similarity).
• Controls lymphocyte response to Helicobacter infection and defense against cytomegalovirus (By similarity).
• Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production. Related references: PMID:23857366
• Subcellularly localized to endoplasmic reticulum, endosomes, lysosomes, and phagosomes, critical for ligand recognition.
• Highly expressed in spleen, lymph node, tonsil, and peripheral blood leukocytes, particularly plasmacytoid pre-dendritic cells.
• Keywords: leucine-rich repeat, transmembrane protein, receptor, immunity, inflammatory response.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within the ectodomain region (aa 414-543), suitable for detecting endogenous TLR9 in relevant sample types.
• For WB, the predicted molecular weight is ~116 kDa; consider using appropriate reducing/denaturing conditions and validate with a positive control lysate from spleen or lymph node.
• IHC-P may require optimization of antigen retrieval and blocking steps; validate in tissues with known high expression (e.g., tonsil, spleen).
• Cross-reactivity with mouse and rat is specified; consider verifying performance in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to validated IVD raw materials, including polyclonal antibodies, monoclonal antibody pairs, and bulk ancillary reagents. This anti-TLR9 antibody supports innate immunity and infectious disease research, and we can assist with sourcing rare-target raw materials to accelerate your assay development.