Anti-TLR8 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9NR97

Core Product Specifications and Parameters

Biological Background: TLR8 Function and Localization

• Toll-like receptor 8 (TLR8), also designated CD288, is an endosomal receptor crucial for innate and adaptive immunity. Related references: PMID:25297876 PMID:32433612
• TLR8 controls immune responses by recognizing RNA degradation products processed by RNASET2, including GU-rich single-stranded RNA from viruses such as SARS-CoV-2, SARS-CoV-1, and HIV-1. Related references: PMID:31778653 PMID:33718825
• Upon ligand binding, TLR8 dimerizes, bringing its TIR domains into contact to recruit the adaptor MYD88, initiating downstream signaling. Related references: PMID:23520111 PMID:25599397 PMID:26929371
• The Myddosome signaling complex (IRAK4, IRAK1, TRAF6, TRAF3) then activates NF-kB and IRF7, inducing pro-inflammatory cytokines and interferons. Related references: PMID:16737960 PMID:17932028 PMID:29155428
• TLR8 localizes primarily to endosome membranes, consistent with its role in sensing internalized pathogen-derived nucleic acids.
• Expressed predominantly in myeloid dendritic cells, monocytes, and monocyte-derived dendritic cells, linking it to professional antigen-presenting cell function.
• Post-translational modifications include disulfide bond formation, glycosylation, and ubiquitylation, which may regulate receptor trafficking and signaling.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 849–1041), which encompasses part of the TIR domain; this design may confer reactivity with both full-length and some splice variants.
• For Western blot, the predicted molecular weight is ~120 kDa; use low-percentage gels and prolonged transfer to ensure efficient detection of high-molecular-weight bands.
• As an endosomal receptor, TLR8 detection by immunofluorescence or immunohistochemistry may require permeabilization steps; Triton X-100 or saponin treatment can be considered.
• When testing cross-reactivity in mouse or rat samples, sequence alignment of the immunogen region is advisable, as epitope conservation may vary.
• For ELISA, pairing with a suitable capture antibody or using this product as a detection antibody could be explored; pretesting with recombinant TLR8 protein is recommended to establish assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, combining validated antibody pairs, optimized polyclonal antibodies like this anti-TLR8 pAb, and bulk ancillary reagents to accelerate innate immunity and infectious disease diagnostics development. Our one-stop supply chain bridges rare target sourcing and technical consultation, empowering labs and manufacturers from concept to clinic.