Anti-TLR5 Rabbit Monoclonal Antibody for WB and ELISA - O60602

Core Product Specifications and Parameters

Biological Background: TLR5 Function and Localization

• Toll-like receptor 5 (TLR5), also known as Toll/interleukin-1 receptor-like protein 3 (TIL3), is a member of the TLR family of pattern recognition receptors.
• Functions as a cell-surface pattern recognition receptor (PRR) that recognizes flagellin, a major pathogen-associated molecular pattern (PAMP) from bacterial flagella, triggering innate immune responses. Related references: PMID:29934223
• Upon flagellin binding, recruits intracellular adaptors MYD88 and TRIF, leading to activation of NF-kappa-B and subsequent production of pro-inflammatory cytokines. Related references: PMID:11489966 PMID:20855887
• Plays a critical role in maintaining intestinal homeostasis and shaping the gut microbiota composition by mediating crosstalk between the host epithelium and commensal microbes (By similarity).
• Localizes to the cell membrane, predominantly on the basolateral surface of intestinal epithelial cells, allowing detection of flagellated bacteria that have crossed the epithelial barrier. Related references: PMID:11489966
• Expressed in various tissues including intestinal and lung epithelia, where it contributes to mucosal defense against pathogens. Related references: PMID:11489966 PMID:18490781
• Contains leucine-rich repeats (LRRs) in its extracellular domain, and undergoes post-translational modifications such as glycosylation and phosphorylation; linked to systemic lupus erythematosus (SLE1).

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was generated using a recombinant fusion protein immunogen spanning amino acids 258-404 of human TLR5 (O60602), corresponding to a portion of the extracellular LRR domain. It is recommended for Western blotting (WB) and ELISA applications per the supplier’s specifications.
• For WB, consider using whole cell lysates from human intestinal (e.g., Caco-2) or lung (e.g., A549) epithelial cell lines as positive controls, with an expected band near 98 kDa.
• When performing ELISA, the antibody may be evaluated as a capture or detection reagent; ensure proper pairing with a compatible secondary or detection antibody and validate in relevant biological matrices.
• As with any immunoassay, optimization of working concentrations and buffer conditions is recommended. Include appropriate negative and positive controls to confirm specificity.

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CamelBio offers comprehensive sourcing solutions for IVD manufacturers and researchers seeking high-quality raw materials, including validated monoclonal antibodies like this anti-TLR5 clone. With expertise in innate immunity and infectious disease targets, we provide one-stop access to antibody pairs, optimized monoclonals, bulk ancillary reagents, and rare target sourcing—helping streamline your assay development from concept to clinic.