Anti-TLR3 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15455

Core Product Specifications and Parameters

Biological Background: TLR3 Function and Localization

• Protein identity: Toll-like receptor 3 (TLR3), also known as CD283, is a member of the Toll-like receptor (TLR) family. It is encoded by the TLR3 gene (GeneID: 7098) and exists in alternatively spliced isoforms.
• Core function: Serves as a key component of innate and adaptive immunity by recognizing double-stranded RNA (dsRNA), a molecular signature of viral infection, leading to NF-κB activation and IRF3 nuclear translocation via the TRIF/TICAM1 adapter.
• Gene family: Belongs to the conserved Toll-like receptor family, which includes pattern recognition receptors that detect pathogen-associated molecular patterns.
• Subcellular localization: Localizes to the endoplasmic reticulum membrane, endosome membrane, and early endosome, consistent with its role in sensing internalized viral RNA.
• Post-translational modifications and structural features: Contains leucine-rich repeats, a signal sequence, and transmembrane helix; is modified by glycosylation, disulfide bond formation, phosphorylation, and ubiquitin-like conjugation.
• Tissue specificity: Highly expressed in placenta and pancreas, and in CD11c+ immature dendritic cells. It is the TLR most strongly expressed in the brain, including astrocytes, glia, and neurons.
• Antimicrobial and signaling roles: Upon dsRNA binding, triggers inflammatory responses, cytokine secretion, and antiviral immunity, highlighting its critical role in host defense.

Experimental Guidance and Technical Tips

• The immunogen was designed using amino acids 500–700 of human TLR3, which maps to the extracellular leucine-rich repeat domain implicated in dsRNA binding. This design may enhance detection of the native, full-length receptor.
• For Western blotting, the predicted molecular weight is 104 kDa; however, due to glycosylation and other modifications, the observed band may appear slightly larger. Validate the antibody in your specific sample system.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider testing both frozen and paraffin-embedded sections, and optimize antigen retrieval conditions as needed.
• The antibody cross-reacts with mouse and rat TLR3, making it suitable for studies in these common model organisms, but confirmation of reactivity in your specific experimental context is recommended.
• For ELISA applications, the polyclonal nature may provide enhanced sensitivity through recognition of multiple epitopes; however, use appropriate controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality reagents like this anti-TLR3 polyclonal antibody. With a focus on innate immunity and infectious disease targets, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinic. Our extensive supply network ensures reliable access to critical raw materials for immunodiagnostic and research applications.