Anti-TLR2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O60603

Core Product Specifications and Parameters

Biological Background: TLR2 Function and Localization

• Cooperates with TLR1 or TLR6 to mediate innate immune responses to bacterial lipoproteins or lipopeptides. Related references: PMID:17889651 PMID:21078852
• Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion, and the inflammatory response.
• May activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins. Related references: PMID:10426995 PMID:10426996
• Recognizes microbial components like MALP-2, STF, PSM, and OspA-L cooperatively with TLR6. Related references: PMID:11441107
• Mediates MAPK activation in response to bacterial peptidoglycan. Related references: PMID:16622205
• Serves as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, modulating antigen presenting cell functions. Related references: PMID:19362712
• Ligand-dependent receptor clustering: TLR2:TLR6:CD14:CD36 for diacylated lipopeptides and TLR2:TLR1:CD14 for triacylated lipopeptides, with subsequent targeting to the Golgi. Related references: PMID:16880211
• Localizes to the cell membrane, cytoplasmic vesicles, phagosome membrane, and membrane rafts. Highly expressed in peripheral blood leukocytes, especially monocytes, bone marrow, lymph node, and spleen.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular TIR domain (aa 636–784 of human TLR2), a region conserved across species, supporting the reported cross-reactivity with mouse and rat.
• For Western blot (WB), expect a band at ~90 kDa. Use appropriate positive controls (e.g., THP-1 or PBMC lysates) and consider validating specificity via blocking peptides or siRNA knockdown.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization is required to detect intracellular TLR2. Optimize fixation and antibody dilution for your cell type.
• The antibody has been used in ELISA; when developing sandwich ELISAs, pairing with a capture antibody against a different epitope may improve sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines access to IVD raw materials, technical services, and consulting—from concept to clinic—for diagnostic manufacturers, laboratories, and researchers. This anti-TLR2 polyclonal antibody supports innate immunity and infection research applications. We also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development.