Anti-TLR2 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - O60603

Core Product Specifications and Parameters

Biological Background: TLR2 Function and Localization

• TLR2 cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins, activating NF-kB via MYD88 and TRAF6. Related references: PMID:10426995 PMID:10426996 PMID:17889651 PMID:21078852
• Recognizes mycoplasmal MALP-2, soluble tuberculosis factor (STF), phenol-soluble modulin (PSM), and B.burgdorferi OspA-L cooperatively with TLR6. Related references: PMID:11441107
• Responds to M.tuberculosis lipoproteins LprA, LprG, LpqH, and PstS1, modulating antigen presenting cell functions. Related references: PMID:19362712
• M.tuberculosis HSP70 (dnaK) stimulates NF-kB expression via TLR2. Related references: PMID:15809303
• MAPK activation in response to bacterial peptidoglycan occurs via TLR2. Related references: PMID:16622205
• Forms activation clusters: TLR2:TLR6:CD14:CD36 for diacylated lipopeptides and TLR2:TLR1:CD14 for triacylated lipopeptides, targeted to the Golgi. Related references: PMID:16880211
• Subcellular localization includes membrane, cytoplasmic vesicle, phagosome membrane, and membrane raft.
• Highly expressed in peripheral blood leukocytes, monocytes, bone marrow, lymph node, and spleen; also detected in lung and fetal liver.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to the extracellular domain (amino acids 290–390) of human TLR2, which may contribute to the antibody’s specificity. Consider this when designing experiments.
• This antibody is suitable for WB, IF/ICC, IF-P, and ELISA. Optimize dilutions and conditions for your specific assay and sample type.
• Cross-reactivity is reported for human and mouse; validate performance in your target species and tissue.
• The predicted molecular weight is ~90 kDa; confirm migration in your system, as post-translational modifications may affect mobility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw materials, technical services, and consulting—from concept to clinic. This TLR2 antibody supports innate immunity research and is backed by CamelBio’s capability to supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Streamline your development with dependable, research-ready raw materials.