Anti-TLR1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15399

Core Product Specifications and Parameters

Biological Background: TLR1 Function and Localization

• Encodes Toll-like receptor 1 (TLR1), also known as CD281 or TIL, a member of the Toll-like receptor family that plays a critical role in pathogen recognition and activation of innate immunity.
• Cooperates with TLR2 to recognize bacterial lipoproteins; specifically, the TLR2:TLR1:CD14 activation cluster detects triacylated lipopeptides, initiating downstream signaling. Related references: PMID:21078852 PMID:16880211
• Signaling occurs through the adaptor MYD88 and TRAF6, leading to NF-kappa-B activation and the secretion of pro-inflammatory cytokines.
• Localizes to the cell membrane and is also found in cytoplasmic vesicles, phagosome membranes, membrane rafts, and the Golgi apparatus, facilitating immune surveillance.
• Ubiquitously expressed with highest levels in spleen, ovary, peripheral blood leukocytes, thymus, and small intestine.
• Contains leucine-rich repeats, a transmembrane domain, and a TIR domain; post-translational modifications include glycosylation and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 30-290), suggesting the antibody may detect both the mature and precursor forms.
• In Western blotting, a band around 90 kDa is expected; due to glycosylation, the observed molecular weight may appear higher.
• For IHC-P, consider optimizing antigen retrieval techniques to achieve robust staining.
• In IF/ICC, expect membrane and vesicular-like staining patterns consistent with reported subcellular localization.
• Cross-reactivity with mouse and rat is indicated, but validation in the relevant species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-TLR1 antibody, bulk ancillary reagents, and rare target sourcing. Our expertise spans innate immunity and inflammatory response research, supporting your projects from concept to clinic.