Anti-TLK1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9UKI8

Core Product Specifications and Parameters

Biological Background: TLK1 Function and Localization

• Serine/threonine-protein kinase tousled-like 1, also known as PKU-beta or Tousled-like kinase 1, is encoded by the TLK1 gene in humans.
• Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase; this inhibition is cell cycle checkpoint- and ATM-pathway dependent and appears to regulate chromatin assembly.
• Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin; additionally, it protects cells from ionizing radiation by facilitating the repair of double-stranded breaks.
• In vitro, phosphorylates histone H3 at Ser-10, suggesting a role in chromatin remodeling and transcriptional regulation.
• Localizes to the nucleus, consistent with its functions in DNA damage response and chromatin dynamics.
• Widely expressed in fetal placenta, liver, kidney, and pancreas, but not in heart or skeletal muscle; isoform 3 is ubiquitously expressed in all tissues examined.
• Annotated with keywords including acetylation, ATP-binding, cell cycle, chromatin regulator, coiled coil, DNA damage, kinase, nucleotide-binding, phosphoprotein, and serine/threonine-protein kinase, reflecting its regulatory modifications and functional domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 160–230 of human TLK1, a region that may be conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting, the expected molecular weight is approximately 87 kDa; consider using appropriate gel percentages and transfer conditions for high-molecular-weight proteins.
• In IHC-P, antigen retrieval conditions may need optimization based on tissue type and fixation method; include known positive and negative control tissues.
• For ELISA, validate specificity with recombinant TLK1 protein and appropriate blocking steps to minimize background.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical assays; it may be suitable as a capture or detection reagent in sandwich immunoassays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined one-stop sourcing solution for IVD raw materials. This TLK1 polyclonal antibody supports research in cell cycle regulation, DNA damage response, and chromatin biology. Beyond individual antibodies, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—empowering your projects from concept to clinic.