Anti-TLK1 Polyclonal Antibody for WB, ELISA - Q9UKI8

Core Product Specifications and Parameters

Biological Background: TLK1 Function and Localization

• TLK1 (Tousled-like kinase 1), also known as PKU-beta, is a serine/threonine-protein kinase.
• It is rapidly and transiently inhibited by phosphorylation following DNA double-stranded breaks during S-phase, in a cell cycle checkpoint and ATM-pathway dependent manner, regulating chromatin assembly.
• In vitro, TLK1 phosphorylates histone H3 at Ser-10, suggesting a role in chromatin modification.
• Isoform 3 phosphorylates and stabilizes the t-SNARE SNAP23, enhancing its assembly with syntaxin, and also facilitates repair of DNA double-stranded breaks.
• TLK1 is primarily localized in the nucleus.
• It is widely expressed, present in fetal placenta, liver, kidney, and pancreas, but not in heart or skeletal muscle; isoform 3 is ubiquitously expressed.
• Key functional keywords include kinase, chromatin regulator, DNA damage, cell cycle, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 150-250 of human TLK1, a region that may be conserved across species; validation in the relevant sample system is recommended.
• This antibody is suitable for Western blot (WB) and ELISA; for other applications, optimization and validation are advised.
• Consider using appropriate controls for DNA damage induction studies, such as ionizing radiation or genotoxic agents, to observe TLK1 phosphorylation dynamics.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-TLK1 polyclonal antibody is part of our extensive portfolio, supporting research into DNA damage response, chromatin biology, and cell cycle regulation. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development and scale-up.