Anti-TLE4 Monoclonal Antibody for WB and ELISA - Q04727

Core Product Specifications and Parameters

Biological Background: TLE4 Function and Localization

• TLE4, also known as Transducin-like enhancer protein 4 or Grg-4, is a transcriptional corepressor that interacts with various transcription factors to inhibit gene expression.
• It functions as a key repressor in the Wnt signaling pathway by binding to CTNNB1 and TCF family members, thereby blocking target gene activation.
• TLE4 plays a critical role in developmental processes, including retina and lens formation, through its essential cooperation with SIX3 for transcriptional auto-repression.
• The protein also mediates transcriptional repression of hormone-related genes such as GNRHR and enhances MSX1-mediated repression of CGA/alpha-GSU.
• TLE4 is predominantly localized in the nucleus, consistent with its role in transcriptional regulation.
• It is subject to post-translational modifications including acetylation and phosphorylation, and contains WD repeat domains important for protein-protein interactions.
• Expression is detected in all tissues examined, with highest levels in brain and muscle, suggesting broad regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-138) of human TLE4, which may confer specificity to the antibody; consider sequence alignment for species cross-reactivity.
• For Western blotting, TLE4 has a predicted molecular weight of approximately 84 kDa; use an appropriate gel percentage (e.g., 8-10% SDS-PAGE) and ensure proper transfer of high molecular weight proteins.
• Validate the antibody's performance in the relevant sample system using positive controls such as human or mouse cell lysates known to express TLE4 (e.g., brain or muscle tissues).
• For ELISA applications, consider pairing with a detection antibody targeting a distinct epitope to avoid competition, and use recombinant TLE4 protein as a standard for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with reliable access to a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This high-quality anti-TLE4 antibody supports research in transcriptional regulation, developmental biology, and Wnt signaling, and can be seamlessly integrated into your assay development pipeline. As your one-stop sourcing bridge, we also specialize in rare target raw-material procurement to accelerate your projects from concept to clinic.