Anti-TLE2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q04725

Core Product Specifications and Parameters

Biological Background: TLE2 Function and Localization

• TLE2 (Transducin-like enhancer protein 2), also known as ESG2, belongs to the groucho family of transcriptional corepressors and shares homology with Drosophila groucho.
• It functions as a transcriptional corepressor by binding to various transcription factors, thereby inhibiting target gene expression.
• TLE2 negatively regulates Wnt signaling by inhibiting the transcriptional activation mediated by CTNNB1 and TCF family members.
• The protein contains WD repeat domains, which facilitate protein-protein interactions essential for its repressor functions.
• TLE2 is primarily localized to the nucleus, consistent with its role in transcriptional regulation.
• It is expressed in all tissues, with highest levels in heart, brain, and muscle, suggesting broad regulatory roles.
• Post-translational modifications include phosphorylation and ubiquitination, which may modulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-138), which is distal to the WD repeat domain; verify epitope accessibility in your experimental context.
• For Western blot, a band around 80 kDa is expected; consider using appropriate gel percentages and transfer conditions for high molecular weight proteins.
• In IHC-P, optimize antigen retrieval methods and antibody dilution; positive nuclear staining should be observed in tissues with known TLE2 expression.
• For ELISA, this antibody can be used as a capture or detection reagent; pair it with a compatible detection system and validate linearity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to a broad portfolio of IVD raw materials, including optimized monoclonal antibodies like this anti-TLE2 clone for Wnt signaling studies. Beyond single reagents, we provide validated antibody pairs, bulk ancillary reagents, and rare target sourcing to support your entire development workflow—from assay concept to clinical application.