Anti-TLE1 Rabbit Polyclonal Antibody for WB and ELISA - Q04724

Core Product Specifications and Parameters

Biological Background: TLE1 Function and Localization

• TLE1 (Transducin-like enhancer protein 1), also known as ESG1 or GRG1, is a member of the TLE/Groucho family of transcriptional corepressors.
• Functions as a transcriptional corepressor by interacting with various transcription factors, including NF-kappa-B, FOXA2, and the CTNNB1/TCF complex in Wnt signaling, thereby inhibiting target gene expression.
• Enhances transcriptional repression mediated by FOXG1/BF-1 and HES1, and can also act as a coactivator for ESRRG, indicating a context-dependent regulatory role.
• Localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• Expressed ubiquitously across human tissues, with higher expression levels observed in brain, liver, and muscle.
• Contains WD repeat domains, which facilitate protein-protein interactions essential for its corepressor function, and is subject to phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 501–770 in the C-terminal region; consider this epitope location when interpreting binding data or using truncated constructs.
• For Western blot, standard SDS-PAGE protocols should yield a band near the predicted 83 kDa; validate the antibody in your target cell lysate to confirm specificity.
• In ELISA applications, titrate the antibody under your specific assay conditions to determine the optimal working dilution for capture or detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD development by offering a comprehensive portfolio of raw materials, including validated polyclonal antibodies like this TLE1 antibody. We support transcription and Wnt signaling research with optimized antibody pairs, ancillary reagents, and sourcing services for rare targets. Partner with CamelBio to efficiently bridge the gap between concept and clinic.