Anti-TLE1 Polyclonal Antibody for WB and ELISA - Q04724

Core Product Specifications and Parameters

Biological Background: TLE1 Function and Localization

• Transducin-like enhancer protein 1 (TLE1), also known as enhancer of split groucho-like protein 1 (ESG1) or GRG1, is a member of the TLE family of transcriptional corepressors.
• It functions as a transcriptional corepressor by interacting with various transcription factors, including NF-κB, FOXA2, and TCF/LEF family members in the Wnt signaling pathway, thereby inhibiting target gene expression.
• TLE1 also exhibits an unusual coactivator function for ESRRG, expanding its regulatory roles.
• The protein localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• TLE1 is expressed in all human tissues examined, with the highest levels found in brain, liver, and muscle.
• It contains multiple WD repeat domains that mediate protein-protein interactions essential for its corepressor/coactivator functions.
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation, which may modulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (amino acids 256–355) of human TLE1, which is conserved and likely to detect the full-length protein in multiple species (validated cross-reactivity with mouse).
• For Western blotting, use whole-cell or nuclear lysates from human or mouse cell lines; the expected band size is approximately 83 kDa.
• ELISA applications may benefit from coating the recombinant immunogen or cell lysates; consider optimizing coating conditions and antibody dilution.
• When studying TLE1 in signaling pathways (e.g., Wnt or NF-κB), validate knockdown or overexpression models to confirm antibody specificity.
• Store the antibody according to recommended conditions (shipped on ice bags) and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality antibodies like this anti-TLE1 polyclonal antibody for studies in transcriptional regulation and cancer biology. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to streamline your assay development. Whether you need rare-target reagents or large-scale production support, CamelBio’s procurement expertise ensures reliable access to the materials that advance your diagnostic pipeline.