Anti-TLE1 Monoclonal Antibody for WB, IHC-P, ELISA - Q04724

Core Product Specifications and Parameters

Biological Background: TLE1 Function and Localization

• TLE1, also known as Transducin-like enhancer protein 1 or enhancer of split groucho-like protein 1 (ESG1), is a member of the Groucho/TLE family of transcriptional corepressors.
• It functions as a transcriptional corepressor that binds to a variety of transcription factors, inhibiting NF-kappa-B-regulated gene expression, and repressing the transcriptional activation mediated by FOXA2 and by CTNNB1/TCF in the Wnt signaling pathway.
• Unusually, TLE1 can also act as a transcriptional coactivator for the nuclear receptor ESRRG, highlighting its context-dependent regulatory roles.
• The repressive effects of full-length TLE family members may be modulated through association with the dominant-negative protein AES.
• TLE1 is predominantly localized in the nucleus, where it mediates its transcriptional regulatory functions.
• Expression is detected in all tissues examined, with highest levels observed in the brain, liver, and muscle.
• The protein features multiple WD repeat domains and is subject to post-translational modifications including phosphorylation and ubiquitin-like conjugation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the internal region (aa 150–250) of human TLE1; consider using a blocking peptide to confirm antibody specificity in your assay.
• For Western blotting, TLE1 has a predicted molecular weight of ~83 kDa; ensure adequate separation and transfer conditions, and consider nuclear-enriched lysates if the signal is weak.
• In immunohistochemistry (IHC-P), validate staining with appropriate positive (e.g., brain or liver tissue) and negative controls, as TLE1 is widely expressed but may show tissue-specific patterns.
• ELISA applications may be optimized by titrating the antibody against the immunogen peptide or recombinant TLE1 protein to establish sensitivity and dynamic range.
• Given the cross-reactivity with mouse and rat, the antibody can be used for interspecies studies, but validate specificity independently in each species due to potential sequence variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research labs, covering every stage from concept to clinic. Supported by deep expertise in transcriptional regulation and cell signaling targets, we offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Our TLE1 monoclonal antibody provides a reliable foundation for assays in gene expression, cancer biology, and developmental signaling—backed by flexible supply solutions that simplify your workflow.