Anti-TKT/Transketolase Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P29401

Core Product Specifications and Parameters

Biological Background: TKT/Transketolase Function and Localization

• Catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate in the pentose phosphate pathway (PubMed:20667822, PubMed:27259054, PubMed:31534226). Related references: PMID:20667822, PMID:27259054, PMID:31534226
• Requires thiamine pyrophosphate (TPP) as a cofactor; forms a covalent intermediate with TPP during catalysis.
• Can also utilize fructose-6-phosphate as a ketose donor, potentially linking glycolysis to the pentose phosphate pathway.
• Exists as a homodimer; three-dimensional structure has been resolved, with each subunit binding metal ions such as Ca²⁺ and Mg²⁺.
• Subject to multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitin-like conjugation (isopeptide bond).
• Mutations in TKT cause a short stature, developmental delay, and congenital heart defects syndrome known as SDDHD (Short Stature, Developmental Delay, and Congenital Heart Defects).
• Alternatively spliced transcript variants produce multiple isoforms with potentially distinct regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 281–380 of human TKT, a region that may represent a surface-accessible epitope in the native homodimer. For immunoprecipitation or flow cytometry, consider gentle lysis conditions to preserve native conformation.
• For Western blot, the predicted band is ~68 kDa; use appropriate gel percentages (e.g., 8–10% SDS-PAGE) and validate antibody dilutions with a relevant positive control lysate (e.g., human, mouse, or rat).
• In IHC-P and IF/ICC applications, standard antigen retrieval and blocking steps are recommended; cross-reactivity with mouse and rat tissue should be validated in your specific experimental context.
• Assess optimal working concentrations individually for ELISA and other applications; batch-to-batch consistency is typically high with recombinant monoclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering validated antibody pairs, monoclonal and polyclonal reagents, and bulk ancillary supplies—all through a single source. From discovery to diagnostic kit assembly, we simplify procurement for pentose phosphate pathway and metabolic research, ensuring timely access to high-quality materials like this TKT/Transketolase antibody. Our consultative approach helps manufacturers navigate technical challenges while staying focused on delivering reliable diagnostic solutions.