Anti-TIRAP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P58753

Core Product Specifications and Parameters

Biological Background: TIRAP Function and Localization

• TIRAP (Toll/interleukin-1 receptor domain-containing adapter protein), also known as MAL (MyD88 adapter-like) or MyD88-2, is an innate immune adaptor protein involved in TLR2, TLR4, and RAGE signaling pathways.
• It functions downstream of these receptors, coupling to IRAK2 and TRAF6 to activate NF-κB, MAPK1/3 (ERK1/2), and JNK, thereby promoting pro-inflammatory cytokine production such as TNF and IL-6.
• TIRAP localizes to the cytoplasm and cell membrane, enabling signal transduction from activated receptors.
• The protein is expressed broadly, with high levels in liver, kidney, spleen, skeletal muscle, and heart, and also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon, and brain.
• Post-translational modifications include phosphorylation, disulfide bond formation, and ubiquitin conjugation (Ubl), which may regulate its activity and interactions.
• Alternative splicing of the TIRAP gene generates multiple isoforms, as indicated by the "Alternative splicing" keyword.
• TIRAP is a key component of the MyD88-dependent signaling branch in innate immunity, and its dysfunction is associated with altered inflammatory responses.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human TIRAP protein (amino acids 1-221), which may provide broad epitope coverage. Consider using this antibody for detecting endogenous TIRAP in various cell lines or tissues.
• For Western blotting, the predicted molecular weight is 24 kDa; use appropriate gel percentage and verify with positive control lysates (e.g., from tissues with high expression like liver or kidney).
• In immunofluorescence/ICC, expect cytoplasmic and membrane staining. Include appropriate controls and consider co-staining with organelle markers.
• The antibody cross-reacts with mouse and rat TIRAP; validate the species-specific detection in the relevant sample system.
• For ELISA applications, optimize coating and detection conditions using the recombinant protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, supporting manufacturers and researchers from concept to clinic. This anti-TIRAP polyclonal antibody, suitable for innate immunity and inflammatory disease research, is part of a broad portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare or custom target reagents, our team assists in bridging procurement gaps efficiently.