Anti-TIRAP Rabbit Polyclonal Antibody for IHC-P, ELISA - P58753

Core Product Specifications and Parameters

Biological Background: TIRAP Function and Localization

• TIRAP (Toll/interleukin-1 receptor domain-containing adapter protein), also known as Mal (MyD88 adapter-like protein), is an adapter protein crucial in innate immune signaling.
• Functions as an adapter in TLR2, TLR4 and RAGE signaling pathways, acting via IRAK2 and TRAF-6 to activate NF-kappa-B, MAP kinases (MAPK1/3 and JNK), leading to cytokine secretion and inflammatory response.
• Positively regulates the production of pro-inflammatory cytokines TNF-alpha and interleukin-6.
• Encoded by the TIRAP (MAL) gene (GeneID: 114609) in humans.
• Subcellularly localized to the cytoplasm, cell membrane, and membrane-associated compartments.
• Highly expressed in liver, kidney, spleen, skeletal muscle, and heart; also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon, and brain.
• Post-translational modifications include phosphorylation (phosphoprotein), disulfide bond formation, and ubiquitination (based on UniProt keywords).
• Classified under keywords: 3D-structure, Alternative splicing, Disulfide bond, Immunity, Inflammatory response, Innate immunity, Phosphoprotein, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human TIRAP (aa 1–221), which should generate polyclonal antibodies recognizing multiple epitopes across the native protein; consider this broad recognition for applications requiring total TIRAP detection.
• Validated for IHC-P and ELISA by the supplier; for IHC-P, consider using tissue sections from TIRAP-high organs such as liver, kidney, or spleen. For ELISA, optimize coating and detection conditions in your system.
• Designed for human TIRAP; cross-reactivity with other species is not reported. If applied to other species, validate specificity based on sequence homology.
• Perform appropriate controls (e.g., blocking peptide, if available) and titrate the antibody for each new application or sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined, one-stop solution for IVD raw materials, technical services, and consulting—from concept to clinic. This anti-TIRAP rabbit polyclonal antibody supports research in innate immunity and inflammatory disease, backed by CamelBio's expertise in sourcing validated antibody pairs, monoclonal and polyclonal antibodies, bulk reagents, and rare-target raw materials. Whether you need off-the-shelf tools or custom development, CamelBio bridges your reagent needs to accelerate IVD development.