Anti-TIRAP Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P58753

Core Product Specifications and Parameters

Biological Background: TIRAP Function and Localization

• TIRAP (Toll/interleukin-1 receptor domain-containing adapter protein), also known as MAL (MyD88 adapter-like protein), is a key adapter in innate immune signaling.
• It mediates signaling downstream of TLR2, TLR4, and RAGE receptors, linking them to downstream effectors.
• Upon receptor activation, TIRAP recruits IRAK2 and TRAF-6, leading to activation of NF-kappa-B and MAP kinases (ERK1/2, JNK), thus promoting transcription of pro-inflammatory cytokines such as TNF and IL-6.
• TIRAP is localized both in the cytoplasm and at the cell membrane, consistent with its role in bridging membrane receptors to intracellular signaling cascades.
• The protein undergoes post-translational modifications, including phosphorylation and ubiquitin-like conjugation, which may regulate its activity and interactions.
• TIRAP is broadly expressed, with highest levels in liver, kidney, spleen, skeletal muscle, and heart, and detectable in peripheral blood leukocytes, lung, and other tissues.
• The TIRAP gene produces multiple isoforms through alternative splicing, potentially fine-tuning immune responses.

Experimental Guidance and Technical Tips

• For Western blot, a specific band around 24 kDa is expected; consider using appropriate positive controls (e.g., cell lines known to express TIRAP) and validate in your sample system.
• The immunogen corresponds to the N-terminal region (amino acids 1–100); ensure the target epitope is conserved across species (human, mouse, rat) when working with cross-reactive samples.
• In IHC-P, optimize antigen retrieval and antibody dilution; high-expressing tissues such as liver or kidney can serve as robust positive controls.
• ELISA can be employed to quantify TIRAP in lysates or purified protein; validate detection using recombinant TIRAP protein.
• Given TIRAP's role in innate immunity, stimulation with TLR agonists (e.g., LPS) may upregulate TIRAP levels in experimental models.

CamelBio: Your One-Stop Sourcing Bridge

Complement your innate immunity research with CamelBio’s comprehensive IVD raw-material portfolio, including validated antibody pairs, recombinant proteins, and ancillary reagents. We specialize in streamlining procurement for diagnostic manufacturers, from monoclonal antibodies against signaling adapters like TIRAP to custom development support. Reach out to explore bulk options or tailored solutions that meet your assay development needs.