Anti-TIR1 Polyclonal Antibody for WB and ELISA - Q570C0

Core Product Specifications and Parameters

Biological Background: TIR1 Function and Localization

• TIR1 (TRANSPORT INHIBITOR RESPONSE 1), also known as weak ethylene-insensitive protein 1 (WEI1), is an auxin receptor that functions as the substrate recognition component of the SCF(TIR1) E3 ubiquitin ligase complex.
• It mediates the ubiquitin-dependent proteasomal degradation of Aux/IAA transcriptional repressors, thereby regulating auxin-responsive gene transcription.
• TIR1 plays a central role in auxin-mediated developmental processes including root and hypocotyl growth, lateral root formation, cell elongation, and gravitropism.
• The protein is localized to the nucleus, consistent with its role in transcriptional regulation.
• It is broadly expressed in roots, stems, leaves, and flowers, with strong expression in floral stigma, anther filaments, abscission zones, and vascular tissues of adult plants.
• Beyond auxin signaling, TIR1 also participates in ethylene signaling in roots and contributes to plant defense by conferring sensitivity to the bacterial pathogen Pseudomonas syringae.
• The three-dimensional structure of TIR1 has been determined, providing insights into auxin recognition and signaling mechanisms.

Experimental Guidance and Technical Tips

• The antibody targets a C-terminal peptide region (amino acids 495–594) of Arabidopsis TIR1, enhancing specificity for the full-length 67 kDa protein.
• In Western blot (WB) assays using Arabidopsis thaliana lysates, a signal at approximately 67 kDa is anticipated.
• For ELISA applications, consider optimizing coating conditions using either recombinant protein or the immunizing peptide.
• As a polyclonal preparation, lot-to-lot variability may occur; validate performance in your assay system before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering not only this TIR1 polyclonal antibody but also a wide range of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support plant biology and signaling research. Our supply capabilities extend to rare target raw materials and customized sourcing solutions, helping you streamline your diagnostics development workflow.