Anti-TIPARP Rabbit Polyclonal Antibody for WB, ELISA - Q7Z3E1

Core Product Specifications and Parameters

Biological Background: TIPARP Function and Localization

• TIPARP (PARP7/ARTD14) is a mono-ADP-ribosyltransferase that catalyzes the transfer of a single ADP-ribose unit from NAD+ onto glutamate, aspartate, and cysteine residues of target proteins. Related references: PMID:23275542, PMID:25043379, PMID:30373764
• Acts as a negative feedback regulator of the aryl hydrocarbon receptor (AHR) signaling pathway by mono-ADP-ribosylating AHR, which suppresses its transcriptional activity. Related references: PMID:23275542, PMID:30373764
• Belongs to the poly(ADP-ribose) polymerase (PARP) family, characterized by a conserved catalytic domain and zinc-finger motifs; the gene is inducible by TCDD (dioxin).
• Localizes predominantly to the nucleus, consistent with its role in modifying nuclear targets.
• Contains a zinc-finger domain and is classified under transferases, glycosyltransferases, and nucleotidyltransferases; requires metal binding for activity.
• May play a role in cellular response to environmental toxins and stress through its regulation of AHR.
• Molecular weight of the unmodified human protein is approximately 76 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 441–657 of human TIPARP, encompassing part of the catalytic domain. This region is expected to be immunogenic; verify reactivity with your sample matrix.
• For Western blot (WB), a band near 76 kDa is anticipated in human and mouse lysates. Optimize primary antibody dilution and detection conditions.
• ELISA applications may require titration of capture/detection antibodies and validation of recombinant or native antigen binding.
• As a rabbit polyclonal antibody, it provides broad epitope recognition but may require additional controls for specificity in complex samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research laboratories with a complete range of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this TIPARP antibody, and bulk ancillary reagents. With capabilities in rare target sourcing, technical consulting, and scalable supply, CamelBio streamlines assay development from concept to clinic, especially for ADP-ribosylation and AHR pathway research.