Anti-TIN2/TINF2 Rabbit Monoclonal Antibody for WB, ELISA - Q9BSI4

Core Product Specifications and Parameters

Biological Background: TIN2/TINF2 Function and Localization

• TIN2, also known as TERF1-interacting nuclear factor 2 or TRF1-interacting nuclear protein 2, is encoded by the TINF2 gene. Alternative splicing produces isoforms 1 and 2.
• TIN2 is a core component of the shelterin (telosome) complex, which binds double-stranded telomeric TTAGGG repeats to safeguard chromosome ends.
• The shelterin complex protects telomeres from being recognized as DNA damage, preventing inappropriate activation of DNA repair pathways at chromosome termini.
• TIN2 facilitates the assembly of the shelterin complex and may contribute to telomere length regulation and protection.
• Isoform 1 has been proposed to play an additional role in tethering telomeres to the nuclear matrix.
• Subcellular localization: TIN2 is primarily found in the nucleus, at telomeres, and possibly associated with the nuclear matrix.
• Tissue expression: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• TIN2 undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitin conjugation (isopeptide bond). Mutations in TINF2 are associated with dyskeratosis congenita and related telomere biology disorders.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 210–309 of human TIN2/TINF2; confirm sequence conservation across human, mouse, and rat to support cross-reactivity expectations.
• For Western blotting, consider using whole-cell lysates or nuclear extracts from human, mouse, or rat cell lines; the predicted molecular weight is ~50 kDa (UniProt: 50023 Da).
• Validate the antibody in the relevant sample system and include appropriate positive and negative controls, such as siRNA-mediated knockdown lysates or known TIN2-expressing cell lines.
• For ELISA, consider coating with recombinant TIN2 protein or cell lysates; optimal antibody dilution should be determined empirically.
• Due to potential isoforms and post-translational modifications, multiple bands may be observed in some samples; confirm specificity with mass spectrometry or detection of tagged TIN2 overexpressors.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined, one-stop sourcing solution for IVD raw materials, from concept to clinic. Our portfolio includes validated monoclonal and polyclonal antibodies, antibody pairs, and bulk ancillary reagents tailored for telomere biology and shelterin complex studies. We also specialize in sourcing rare target raw materials, supporting your assay development with a reliable supply chain.