Anti-TIN2/TINF2 Polyclonal Antibody for WB, ELISA - Q9BSI4

Core Product Specifications and Parameters

Biological Background: TIN2/TINF2 Function and Localization

• TIN2 (TERF1-interacting nuclear factor 2), also known as TRF1-interacting nuclear protein 2, is encoded by the TINF2 gene and exists as multiple isoforms (isoform 1 canonical).
• It is a core component of the shelterin complex, which binds telomeric double-stranded TTAGGG repeats to protect chromosome ends from DNA damage repair pathways and regulate telomere length.
• TIN2 plays a key role in the assembly of the shelterin complex, bridging TRF1 and TRF2 with other components, and isoform 1 may tether telomeres to the nuclear matrix.
• The protein localizes to the nucleus, specifically at chromosome telomeres, and is also associated with the nuclear matrix.
• TIN2 is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitin conjugation, which may regulate its function and interactions.
• Mutations in TINF2 are linked to dyskeratosis congenita, a telomere biology disorder, underscoring its critical role in telomere maintenance.
• Tissue expression is broad, with transcripts detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 202–451 of human TIN2, a region that may capture multiple isoforms; verify detection of expected bands in your sample system.
• For Western blot, the predicted molecular weight is approximately 50 kDa; use appropriate gel percentages and transfer conditions.
• As a polyclonal antibody, it may exhibit lot-to-lot variation; include positive and negative controls in each experiment.
• Cross-reactivity with mouse allows for comparative studies; however, confirm reactivity in your specific mouse strain or tissue.
• For ELISA, consider the immunogen design when setting up capture/detection formats, and optimize coating and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a dedicated one-stop IVD raw-material partner, connecting diagnostic developers with high-quality reagents like this anti-TIN2 polyclonal antibody for telomere biology and shelterin complex research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development from concept to clinic. Let us help you secure the full range of raw materials with reliable supply and technical alignment.