Anti-TIMP4 Polyclonal Antibody for WB, IF/ICC, ELISA - Q99727

Core Product Specifications and Parameters

Biological Background: TIMP4 Function and Localization

• Metalloproteinase inhibitor 4 (TIMP-4) is a member of the tissue inhibitor of metalloproteinases (TIMP) family, which regulate extracellular matrix remodeling by inhibiting matrix metalloproteinases (MMPs).
• TIMP-4 irreversibly inactivates MMPs such as MMP-1, MMP-2, MMP-3, MMP-7, and MMP-9 through binding to their catalytic zinc cofactor.
• It is abundant in cardiac tissue, with lower expression levels detected in many other tissues, reflecting its role in cardiovascular biology.
• The protein is secreted into the extracellular space, where it exerts its inhibitory function on MMPs.
• Structural features include conserved disulfide bonds and a zinc-binding site, typical of the TIMP family, contributing to its stability and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30–224 of human TIMP4, representing the mature secreted form; the antibody is expected to recognize endogenous TIMP4.
• For Western blot analysis, validate in relevant human, mouse, or rat heart tissue lysates; TIMP4 migrates at approximately 26 kDa under reducing conditions.
• For immunofluorescence/immunocytochemistry, test fixation and permeabilization conditions, as TIMP4 is secreted; co-localization studies with MMPs may be informative.
• In ELISA, confirm specificity by competitive binding with recombinant TIMP4 protein; note potential cross-reactivity with other TIMP family members due to the polyclonal nature of the antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies such as this TIMP4 antibody. Whether you require bulk ancillary reagents, rare target reagents, or tailored technical consultation, our one-stop platform supports your projects from concept to clinic. Let us help accelerate your metalloproteinase and cardiovascular disease research with reliable sourcing solutions.