Anti-TIMP2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P16035

Core Product Specifications and Parameters

Biological Background: TIMP2 Function and Localization

• TIMP2 (Metalloproteinase inhibitor 2) is a secreted protein of the tissue inhibitor of metalloproteinases (TIMP) family, also known as CSC-21K.
• It irreversibly inactivates matrix metalloproteinases (MMPs) by binding to their catalytic zinc cofactor.
• TIMP2 inhibits a wide range of MMPs including MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16, and MMP-19.
• By regulating MMP activity, TIMP2 plays a crucial role in extracellular matrix (ECM) remodeling, affecting processes such as tissue repair, angiogenesis, and tumor metastasis.
• The protein is secreted (UniProt) and contains several conserved disulfide bonds that are important for its structural integrity and inhibitory function.
• TIMP2 can also form non-inhibitory complexes, such as with MMP-2 (progelatinase A), modulating cell migration and signaling pathways.
• The immunogen for this antibody is a synthetic peptide spanning amino acids 100-200, which likely maps to a region involved in MMP interaction or protein folding.
• Keywords associated with TIMP2 include: Secreted, disulfide bond, metal-binding, metalloenzyme inhibitor, metalloprotease inhibitor, protease inhibitor, and reference proteome.

Experimental Guidance and Technical Tips

• In western blot, expect a band around 24 kDa, consistent with the calculated molecular weight of TIMP2. Additional bands may appear due to glycosylation or complex formation.
• For immunohistochemistry (paraffin), optimize antigen retrieval using heat-induced epitope retrieval (HIER) in citrate or EDTA buffer; predilution of the antibody may require empirical testing.
• ELISA applications may benefit from pairing this antibody with a capture antibody targeting a distinct epitope; cross-reactivity should be validated in the specific assay matrix.
• Since the antibody cross-reacts with human, mouse, and rat, verify target specificity in your tissue or cell line using positive and negative controls.
• Storage: aliquot and store at -20°C or below; avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, technical services, and consulting. For projects targeting TIMP2 in extracellular matrix remodeling or cancer metastasis, we supply high-quality antibodies and can source ancillary reagents in bulk. Our expertise ranges from antibody pair optimization to rare target procurement, helping you accelerate from concept to clinic.