Anti-TIMP2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16035

Core Product Specifications and Parameters

Biological Background: TIMP2 Function and Localization

• TIMP2 (Metalloproteinase inhibitor 2) is a secreted protein that belongs to the tissue inhibitor of metalloproteinases (TIMP) family, characterized by its ability to inhibit matrix metalloproteinases (MMPs).
• The primary function of TIMP2 is to form non-covalent complexes with various MMPs, including MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16, and MMP-19, thereby irreversibly inactivating them.
• Inhibition occurs through binding of TIMP2 to the catalytic zinc cofactor present in the active site of MMPs, blocking substrate access and enzymatic activity.
• TIMP2 is synthesized as a precursor with a signal peptide and is secreted into the extracellular matrix, where it plays a crucial role in regulating extracellular matrix degradation and tissue remodeling.
• The protein contains multiple disulfide bonds that stabilize its three-dimensional structure, essential for its inhibitory function.
• TIMP2 is classified under keywords such as Metalloprotease inhibitor, Protease inhibitor, and Metal-binding, highlighting its role in controlling proteolysis and zinc-dependent enzymes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 50-150 of human TIMP2, a region likely including functional domains; verify specificity using knockout or knockdown controls in relevant cell lines.
• For Western blot (WB), due to the presence of disulfide bonds, consider using reducing conditions and heating to ensure proper denaturation and detection at the predicted weight of ~24 kDa.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), TIMP2 is secreted; thus, permeabilization may not be necessary, but fixation conditions should be optimized to preserve extracellular epitopes.
• The antibody cross-reacts with mouse and rat TIMP2; validate in each species system individually for your specific application.
• For ELISA, pair with a suitable detection antibody and assess standard curves using recombinant TIMP2 protein to confirm sensitivity and dynamic range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic manufacturers and research laboratories. For studies involving TIMP2 and tissue remodeling, we provide one-stop access to high-quality reagents tailored to your assay development needs, streamlining the path from concept to clinic. Our expertise in rare target sourcing ensures you can obtain the necessary raw materials efficiently, backed by technical consultation at every stage.