Anti-TIMP2 Monoclonal Antibody for WB, ELISA - P16035

Core Product Specifications and Parameters

Biological Background: TIMP2 Function and Localization

• TIMP2 (Metalloproteinase inhibitor 2), also known as CSC-21K or DDC8, is a member of the tissue inhibitor of metalloproteinases (TIMP) family.
• It irreversibly inactivates various matrix metalloproteinases (MMPs) by binding to their catalytic zinc cofactor, inhibiting collagenases and gelatinases.
• Known targets include MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16, and MMP-19.
• The protein is secreted and contains a signal peptide, consistent with its extracellular role in matrix remodeling.
• Structural features include disulfide bonds and a zinc-binding site, critical for its inhibitory function.
• TIMP2 has been identified by proteomics and direct protein sequencing, with a reference proteome record (UniProt: P16035).

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein spanning amino acids 120–220 of human TIMP2; consider using it to detect an epitope within this region.
• For Western blot, run samples under reducing conditions and expect a band at ~24 kDa; validate with appropriate positive and negative controls.
• Since TIMP2 is secreted, assess conditioned media or cell lysates for maximal detection.
• In ELISA, pair with a capture/detection antibody recognizing a non-overlapping epitope to avoid steric interference.
• Reported cross-reactivity includes human, mouse, rat, and African green monkey; verify reactivity in the species and sample type of interest before quantitative studies.

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