Anti-TIMP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P01033

Core Product Specifications and Parameters

Biological Background: TIMP1 Function and Localization

• TIMP1 (Metalloproteinase inhibitor 1) is a secreted protein that functions as a key inhibitor of matrix metalloproteinases (MMPs), including collagenases, by forming 1:1 complexes and irreversibly inactivating them through binding to their catalytic zinc cofactor.
• It acts on a broad range of MMPs such as MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13, and MMP16, but does not inhibit MMP14.
• Beyond MMP inhibition, TIMP1 acts as a growth factor that regulates cell differentiation, migration, and apoptosis, activating cellular signaling cascades via receptors CD63 and ITGB1.
• TIMP1 mediates erythropoiesis in vitro, stimulating the growth and differentiation of human and murine erythroid progenitors in a species-specific manner.
• The protein is also known as Erythroid-potentiating activity (EPA) and Fibroblast collagenase inhibitor, reflecting its dual roles.
• TIMP1 is detected in rheumatoid synovial fluid, indicating involvement in inflammatory joint diseases.
• Post-translational modifications include glycosylation, phosphorylation, and the presence of disulfide bonds, which are critical for its stability and function.
• Structurally, it contains a signal peptide, a zinc-binding motif, and belongs to the tissue inhibitor of metalloproteinases (TIMP) family.

Experimental Guidance and Technical Tips

• For Western blotting, given the protein's ~23 kDa molecular weight and presence of disulfide bonds, consider reducing and denaturing conditions to ensure proper migration.
• For immunofluorescence applications, optimize fixation and permeabilization steps for secreted proteins; co-staining with organelle markers may help confirm localization.
• As the antibody was raised against a recombinant fragment, it is expected to recognize both native and denatured TIMP1; however, validation in the relevant species and sample type is recommended.
• When using in ELISA, pair with an appropriate detection antibody and validate assay conditions, as polyclonal antibodies may exhibit lot-to-lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. For TIMP1-related projects, we provide access to reliable antibody resources and custom sourcing services to accelerate assay development from concept to clinic, particularly in the areas of MMP biology and inflammatory disease.