Anti-TIMM50 Rabbit Monoclonal Antibody for WB, IF, IHC-P, ELISA - Q3ZCQ8

Core Product Specifications and Parameters

Biological Background: TIMM50 Function and Localization

• TIMM50 (also known as TIM50, TIM50L, MGCA9) encodes the mitochondrial import inner membrane translocase subunit TIM50, a critical component of the TIM23 complex.
• It mediates the translocation of nuclear-encoded, transit peptide-containing preproteins across the mitochondrial inner membrane. Related references: PMID:30190335, PMID:38828998
• The protein localizes to the mitochondrial inner membrane and also to nuclear speckles, as indicated by subcellular fractionation and immunofluorescence.
• TIMM50 possesses in vitro phosphatase activity, though this may not be physiologically relevant, and it may participate in the release of snRNPs and SMN from Cajal bodies.
• Widely expressed, with higher protein levels observed in brain, kidney, and liver, suggesting tissue-specific roles in mitochondrial function.
• Mutations in TIMM50 are associated with autosomal recessive epileptic encephalopathy and intellectual disability, highlighting its importance in neuronal homeostasis.
• The protein is subject to alternative splicing, phosphorylation, and has RNA-binding properties, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide covering amino acids 1–100 of human TIMM50, which spans the N-terminal region upstream of the transmembrane domain; this design may allow recognition of multiple isoforms and uncleaved precursor forms.
• For Western blotting, a band at ~40 kDa is expected; consider using mitochondrial enriched fractions or positive control lysates from brain or liver tissues to verify specificity.
• In immunofluorescence and immunohistochemistry, signal is anticipated at mitochondrial networks and possibly nuclear puncta; co-staining with a mitochondrial marker (e.g., Tom20, CoxIV) is recommended to confirm localization.
• The antibody cross-reacts with mouse and rat TIMM50, making it suitable for rodent model studies; validations in the relevant sample system are advised before large-scale experiments.
• If using for ELISA, pairing with a capture or detection antibody against a distinct epitope may enhance assay performance; consider antigen coating optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined one-stop sourcing solution for IVD raw materials. This anti-TIMM50 monoclonal antibody is part of our extensive catalog targeting mitochondrial biology and neurological disease markers, enabling development of high-performance assays. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and custom raw-material sourcing, we support your entire workflow from concept to clinic.