Anti-TIMM44 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43615

Core Product Specifications and Parameters

Biological Background: TIMM44 Function and Localization

• TIMM44 (also known as TIM44) is the mitochondrial import inner membrane translocase subunit TIM44, a crucial component of the protein import machinery.
• It is an essential subunit of the presequence translocase-associated motor (PAM) complex, which drives ATP-dependent translocation of nuclear-encoded mitochondrial proteins from the inner membrane into the matrix.
• TIMM44 recruits mitochondrial HSP70 (mtHsp70) to the translocating polypeptide chain; mtHsp70 binding and ATP hydrolysis provide the driving force for protein import.
• The protein localizes to both the mitochondrial inner membrane and the matrix, bridging the translocon and the import motor.
• According to UniProt annotation, TIMM44 contains a mitochondrial transit peptide, is phosphorylated (phosphoprotein), and possesses ATP/nucleotide-binding motifs, suggesting regulatory roles in import.
• The TIMM44 gene is well-conserved and belongs to the TIMM44 family; it is expressed broadly across human tissues (proteomics identification) and is essential for mitochondrial biogenesis.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody spans amino acids 153-452 of human TIMM44, which corresponds to the mature portion of the protein beyond the mitochondrial targeting sequence, allowing recognition of the processed form.
• For western blotting (WB), a specific band near 51 kDa is expected in lysates from human, mouse, and rat cells or tissues. Appropriate loading controls and molecular weight markers should be included.
• In immunofluorescence (IF/ICC), a mitochondrial staining pattern (filamentous or punctate) is anticipated. Co-staining with a mitochondrial marker (e.g., MitoTracker) is recommended to confirm specificity.
• This antibody is also suitable for ELISA-based detection; consider using recombinant TIMM44 protein as a positive control.
• As with any polyclonal antibody, lot-to-lot variability may occur; optimization of dilution and blocking conditions is advised for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, contract research organizations, and academic labs with streamlined access to a broad portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents like this anti-TIMM44 polyclonal antibody. By focusing on mitochondrial research targets such as TIMM44, CamelBio supports the development of assays for mitochondrial dysfunction studies and related biomarker programs. In addition to standard catalog products, we offer custom antibody generation, bulk supply, and technical consultation to accelerate your projects from concept to clinical application.