Anti-Timm29 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q8BGX2

Core Product Specifications and Parameters

Biological Background: Timm29 Function and Localization

• Timm29 (Translocase of inner mitochondrial membrane 29) is a subunit of the TIM22 complex, a mitochondrial inner membrane translocase responsible for the import and insertion of multi-pass transmembrane proteins.
• The TIM22 complex forms a twin-pore translocase that utilizes the mitochondrial membrane potential as its driving force for protein translocation.
• Timm29 is essential for the stability of the TIM22 complex and facilitates the assembly of the TIMM22 protein into this complex.
• It may facilitate crosstalk between the TIM22 and TOM complexes by interacting with the outer membrane protein TOMM40.
• Subcellular localization: Mitochondrion inner membrane.
• Contains a transit peptide and at least one transmembrane helix; predicted molecular weight ~29.4 kDa (precursor).
• Belongs to a family of mitochondrial import components; orthologs conserved across vertebrates.
• Keywords: Protein transport, translocation, mitochondrial inner membrane.

Experimental Guidance and Technical Tips

• For Western blotting, detect a band around 29 kDa; consider validating in appropriate tissue or cell lysates expressing Timm29.
• For immunoprecipitation, confirm complex formation under native conditions to preserve TIM22 complex integrity.
• The antibody is raised against a central region (amino acids 157-266), which may recognize both the precursor and mature forms of Timm29.
• For ELISA, optimize coating conditions using recombinant Timm29 or cell lysates; cross-reactivity with human and mouse enables comparative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For mitochondrial biology and protein import studies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with CamelBio to accelerate your IVD assay development with reliable, research-grade mitochondrial research reagents.