Anti-TIMM23 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O14925

Core Product Specifications and Parameters

Biological Background: TIMM23 Function and Localization

• TIMM23 (also known as TIM23) is the human ortholog of yeast Tim23, a core component of the TIM23 translocase complex that resides in the mitochondrial inner membrane.
• Essential for the import of nucleus-encoded precursor proteins bearing cleavable N-terminal presequences across the mitochondrial inner membrane (PubMed:10339406). Related references: PMID:10339406
• Plays a critical regulatory role in stress-induced mitophagy by protecting PINK1 from OMA1-mediated degradation, thereby permitting PINK1 accumulation at the outer mitochondrial membrane upon mitochondrial depolarization (PubMed:37160114). Related references: PMID:37160114
• Localizes exclusively to the mitochondrial inner membrane, consistent with its function in protein translocation.
• Predicted to contain multiple transmembrane helices that anchor it in the membrane and likely contribute to the protein-conducting channel of the TIM23 complex.
• Associated with UniProt keywords that highlight its roles in protein transport, translocation, and mitochondrial inner membrane biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region near the N-terminus (amino acids 7–72) of human TIMM23; it is expected to detect endogenous TIMM23 in human, mouse, and rat samples based on sequence homology.
• For Western blotting, a band of approximately 22 kDa is anticipated in whole-cell lysates or mitochondrial fractions from appropriate cell lines (e.g., HeLa, HEK293) or tissues.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing antigen retrieval and permeabilization conditions for mitochondrial membrane proteins; including a mitochondrial marker co-stain can help confirm localization.
• This polyclonal antibody may recognize multiple epitopes; therefore, non-specific bands could appear. Include appropriate controls (e.g., knockdown lysates or blocking peptide) to validate specificity.
• Potential for immunoprecipitation or other applications not listed should be evaluated by the end user as the reagent has not been validated for those uses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for diagnostic manufacturers, CROs, and research institutions seeking high-quality IVD raw materials. For mitochondrial research and TIMM23-related projects, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents (blocking buffers, detection systems), and can facilitate custom sourcing of rare-target raw materials. Our team is ready to support your transition from concept to clinic with competitive lead times and consistent lot-to-lot performance.