Anti-Timm22 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q9CQ85

Core Product Specifications and Parameters

Biological Background: Timm22 Function and Localization

• Mitochondrial import inner membrane translocase subunit Tim22 (Timm22) is an essential core component of the TIM22 complex.
• The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane.
• Timm22 constitutes the voltage-activated and signal-gated channel of the complex.
• It forms a twin-pore translocase that uses the membrane potential as an external driving force in two voltage-dependent steps.
• Subcellular localization is the mitochondrial inner membrane.
• Contains disulfide bonds and transmembrane helices; involved in protein transport and translocation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19–121 of mouse Timm22; verify detection of full-length endogenous protein in your sample system.
• For Western blot, the predicted band is around 20 kDa; mitochondrial-enriched fractions from appropriate tissues may enhance signal.
• The antibody is reported to cross-react with human, mouse, and rat Timm22; perform species-specific validation if required.
• For immunoprecipitation, use cells or tissues with known Timm22 expression; rabbit polyclonal antibodies generally require Protein A/G beads.
• In ELISA applications, use recombinant Timm22 protein or peptide as coating antigen and optimize working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. As your sourcing partner, we support mitochondrial research and protein transport studies by offering high-quality antibodies like anti-Timm22, along with technical services and consulting from concept to clinic.