Anti-TIMM22 Monoclonal Antibody for WB and ELISA - Q9Y584

Core Product Specifications and Parameters

Biological Background: TIMM22 Function and Localization

• TIMM22 encodes the mitochondrial import inner membrane translocase subunit Tim22, also designated testis-expressed protein 4 (TEX4).
• It acts as an essential core component of the TIM22 complex, mediating the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane.
• Tim22 forms the voltage-activated and signal-gated channel of the complex; it constitutes a twin-pore translocase that utilizes the membrane potential as a driving force in two voltage-dependent steps.
• The protein is anchored in the mitochondrion inner membrane and is classified as a multi-pass membrane protein with several transmembrane helices and at least one disulfide bond.
• Disease variants in TIMM22 are associated with combined oxidative phosphorylation deficiency 43 (COXPD43), underscoring its critical role in mitochondrial function.
• The calculated molecular weight is approximately 20 kDa (20031 Da).

Experimental Guidance and Technical Tips

• For Western blot, expect a band near 20 kDa; consider using a 12–15% SDS-PAGE gel for optimal resolution and include a mitochondrial-enriched fraction as a positive control.
• In ELISA, the immunizing peptide (amino acids 20–120) can serve as a convenient positive coating antigen; use peptide competition assays to confirm binding specificity.
• The antibody cross-reacts with human, mouse, and rat TIMM22, so it may be suitable for multi-species studies; nonetheless, species-specific validation is recommended in each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research institutions with streamlined access to IVD raw materials, including this anti-TIMM22 rabbit monoclonal antibody for mitochondrial import studies. Our portfolio encompasses validated antibody pairs, optimized recombinant monoclonal and polyclonal antibodies, bulk ancillary reagents, and dedicated sourcing for rare or difficult-to-find targets. We aim to be your reliable partner in accelerating assay development from initial concept to clinical application.