Anti-TIMM17B Rabbit Polyclonal Antibody for WB, ELISA - O60830

Core Product Specifications and Parameters

Biological Background: TIMM17B Function and Localization

• Essential component of the TIM23 complex, a multimeric assembly that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
• Belongs to the Tim17/Tim22/Tim23 family of inner membrane translocases, sharing a conserved structure critical for protein import.
• Localizes to the mitochondrion inner membrane, where it functions as a transmembrane protein with multiple membrane-spanning helices.
• Tissue expression is abundant in heart and skeletal muscle, intermediate in brain, and weak in pancreas, placenta, kidney, and liver, indicating a role in high-energy-demand tissues.
• Subject to alternative splicing and contains a disulfide bond; participates in protein transport and translocation pathways.
• Identified as a reference proteome component with proteomics identification, supporting its conserved and essential mitochondrial function.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human TIMM17B protein (aa 1-172), suggesting that the antibody may recognize denatured or reduced forms in WB. Consider optimizing sample preparation to maintain protein integrity.
• For WB, validate the antibody in your specific cell or tissue lysate, as expression levels vary significantly across tissues; heart and skeletal muscle are recommended positive controls.
• Cross-reactivity with mouse is confirmed, making it suitable for studies using mouse models. Consider titration to identify the optimal working dilution for your system.
• In ELISA, use a recombinant protein or peptide as coating antigen to assess binding specificity. Pair with secondary detection antibodies appropriate for rabbit IgG.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop access to IVD raw materials, connecting diagnostic manufacturers and research institutions with high-quality antibodies like the TIMM17B Rabbit pAb for mitochondrial biology and protein transport research. Our global supply network provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and efficient sourcing for even rare targets. Partner with us to streamline your assay development and scale-up processes.