Anti-TIMM17B Polyclonal Antibody for WB, ELISA - O60830

Core Product Specifications and Parameters

Biological Background: TIMM17B Function and Localization

• TIMM17B encodes the mitochondrial import inner membrane translocase subunit Tim17-B, a critical component of the TIM23 complex.
• The TIM23 complex specifically mediates the translocation of transit peptide-containing preproteins across the mitochondrial inner membrane, a fundamental process for mitochondrial biogenesis.
• TIMM17B is an integral membrane protein embedded in the mitochondrial inner membrane, featuring multiple transmembrane helices that facilitate its translocase function.
• Tissue expression analysis shows TIMM17B is most abundant in heart and skeletal muscle, with intermediate levels in brain, and weak expression in pancreas, placenta, kidney, and liver.
• Post-translational modifications include alternative splicing and disulfide bond formation, which may influence protein stability and function.
• Functional keywords associated with TIMM17B include protein transport, translocation, membrane, mitochondrion, and alternative splicing, underscoring its role in mitochondrial proteostasis.

Experimental Guidance and Technical Tips

• The immunogen peptide (aa 53-152) targets a central region of TIMM17B, potentially capable of recognizing both native and denatured protein in WB under reducing or non-reducing conditions.
• For ELISA, consider using the peptide immunogen as a coating antigen and optimizing antibody dilutions; note that endogenous TIMM17B detection may require careful lysis and sample preparation.
• Due to high expression in heart and skeletal muscle, these tissues serve as ideal positive controls for antibody validation in human or rat samples.
• Cross-reactivity with rat TIMM17B enables translational studies; however, always verify specificity in your model system with appropriate blocking peptides or knockout controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research institutes with comprehensive IVD raw material sourcing, including validated antibodies for mitochondrial research targets like TIMM17B. Whether you need bulk polyclonal antibodies, matched antibody pairs, or ancillary reagents, we streamline procurement from concept to clinic. Our expertise includes rare target sourcing and customized solutions for mitochondrial biology and protein translocation studies.