Anti-TIMM17B Monoclonal Antibody for WB - O60830

Core Product Specifications and Parameters

Biological Background: TIMM17B Function and Localization

• TIMM17B, also known as Tim17-B, is a subunit of the TIM23 complex, a translocase essential for mitochondrial protein import.
• It functions in the translocation of nuclear-encoded, transit peptide-containing precursor proteins across the mitochondrial inner membrane.
• TIMM17B is an integral membrane protein located in the mitochondrial inner membrane.
• Expression of TIMM17B is tissue-specific, with highest levels in heart and skeletal muscle, moderate in brain, and lower in pancreas, placenta, kidney, and liver.
• The protein features multiple transmembrane helices and a disulfide bond, consistent with its role in membrane translocation.
• Alternative splicing of TIMM17B generates different isoforms, potentially regulating its function or localization.
• As a core component of the TIM23 complex, TIMM17B is involved in essential cellular processes, and its study aids in understanding mitochondrial biogenesis and diseases.
• Keywords from UniProt: Alternative splicing, Disulfide bond, Membrane, Mitochondrion, Mitochondrion inner membrane, Protein transport, Proteomics identification, Reference proteome, Translocation, Transmembrane, Transmembrane helix, Transport.

Experimental Guidance and Technical Tips

• When using this antibody in Western blot (WB), consider appropriate loading controls such as mitochondrial markers (e.g., COX IV) to validate mitochondrial enrichment.
• For ELISA, optimize coating conditions with the immunogen peptide or recombinant protein to ensure specific detection.
• Because TIMM17B is a mitochondrial membrane protein, sample preparation should include methods to preserve membrane integrity or use denaturing conditions as needed.
• Cross-reactivity with mouse and rat suggests conserved epitope recognition; however, validate in the relevant species and tissue system.
• The predicted molecular weight of TIMM17B is ~18 kDa (UniProt: 18273 Da), though post-translational modifications or isoforms may result in band shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your mitochondrial research and assay development needs. Partner with CamelBio for streamlined procurement and tailored solutions in mitochondrial protein import studies.