Anti-TIMM10B Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y5J6

Core Product Specifications and Parameters

Biological Background: TIMM10B Function and Localization

• TIMM10B, also known as FXC1 or Tim9b, is a component of the mitochondrial TIM22 complex, essential for the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane.
• The TIM22 complex functions as a twin-pore translocase that utilizes the mitochondrial membrane potential to drive protein import.
• TIMM10B likely acts as a docking point for the soluble 70 kDa chaperone complex that escorts precursor proteins through the aqueous intermembrane space.
• The protein localizes to the mitochondrial inner membrane.
• TIMM10B is ubiquitously expressed, with highest levels observed in heart, kidney, liver, and skeletal muscle, reflecting its role in tissues with high mitochondrial activity.
• It contains conserved zinc-binding and disulfide bond motifs, characteristic of small Tim proteins, which are important for its structural stability and function.
• Keywords associated with TIMM10B include Metal-binding, Zinc, Disulfide bond, Mitochondrion inner membrane, Protein transport, and Translocation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human TIMM10B protein (amino acids 1-103), ensuring recognition of the native conformation.
• For WB analysis, consider using mitochondrial-enriched fractions from human or mouse heart or skeletal muscle to enhance signal due to high TIMM10B expression in these tissues.
• When performing IHC-P, validate antibody specificity using known positive tissues (e.g., heart, kidney) and include appropriate negative controls (e.g., tissue from TIM22 complex-deficient models).
• Due to the presence of intramolecular disulfide bonds in TIMM10B, reducing conditions in SDS-PAGE may be necessary to resolve distinct bands; however, the predicted molecular weight is approximately 12 kDa.
• ELISA performance can be optimized by using recombinant TIMM10B protein as coating antigen and testing the antibody in both direct and sandwich formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and researchers with streamlined access to high-quality antibodies, antigens, and ancillary reagents. This TIMM10B polyclonal antibody exemplifies our ability to supply rare-target antibodies for mitochondrial and import pathway research. Whether you require bulk quantities, custom immunogen design, or technical consultation, CamelBio bridges the gap from concept to clinic with reliable supply chain solutions.