Anti-TIMM10 Polyclonal Antibody for WB, IF/ICC, ELISA - P62072

Core Product Specifications and Parameters

Biological Background: TIMM10 Function and Localization

• TIMM10 (also known as TIM10, TIM10A, TIMM10A) is a mitochondrial intermembrane chaperone that facilitates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane.
• It acts as a chaperone, protecting hydrophobic precursor proteins from aggregation and guiding them through the mitochondrial intermembrane space.
• TIMM10 is also involved in the transfer of beta-barrel precursors from the TOM complex to the SAM complex in the outer membrane.
• The protein localizes to the mitochondrial inner membrane.
• It contains disulfide bonds and binds zinc, which are important for its structural stability and function.
• TIMM10 is ubiquitously expressed, with highest levels in heart, kidney, liver, and skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human TIMM10 protein (amino acids 1-90), suggesting the antibody may recognize both native and denatured forms. Validate in your specific assay conditions.
• For Western blot (WB), a band of approximately 10 kDa is expected. Consider using appropriate molecular weight markers and optimize antibody dilution for your sample type.
• In immunofluorescence (IF/ICC), mitochondrial localization should be observed. Include appropriate controls to confirm specificity.
• The antibody cross-reacts with mouse and rat, making it suitable for studies in these species. Verify cross-reactivity in your experimental system if using other species.
• For ELISA, the antibody can be used for antigen detection. Titrate the antibody to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-TIMM10 polyclonal antibody as part of its comprehensive IVD raw-material portfolio, ideal for research into mitochondrial protein import and related pathways. Beyond individual antibodies, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing solutions for rare targets, supporting diagnostic manufacturers and research laboratories from concept to clinic.