Anti-TIM44 Rabbit Monoclonal Antibody for WB and ELISA - O43615

Core Product Specifications and Parameters

Biological Background: TIM44 Function and Localization

• TIM44 (TIMM44) is a subunit of the mitochondrial import inner membrane translocase, playing a key role in the presequence translocase-associated motor (PAM) complex.
• It is an essential component required for the ATP-dependent translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix.
• TIM44 recruits mitochondrial Hsp70 (mtHsp70) to the import machinery, facilitating protein import through ATP hydrolysis.
• The protein localizes to the mitochondrial inner membrane and is also associated with the mitochondrial matrix.
• TIM44 features ATP-binding and nucleotide-binding domains and is classified as a phosphoprotein involved in protein transport.

Experimental Guidance and Technical Tips

• This antibody was generated against a recombinant fragment spanning amino acids 10-240 of human TIM44, covering a region critical for interaction with mtHsp70.
• For Western blotting, consider using mitochondrial-enriched lysates and verify a specific band at approximately 51 kDa.
• In ELISA applications, optimal coating conditions and antibody dilutions should be empirically determined.
• Given cross-reactivity with rat, validate performance in the relevant rodent tissue or cell line before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes by providing streamlined access to IVD raw materials. For mitochondrial biology and import pathway studies, CamelBio offers validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to accelerate assay development. Our sourcing network ensures reliable delivery of rare-target materials like TIM44 antibodies, helping you advance from concept to clinic.