Anti-TIM23 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - O14925

Core Product Specifications and Parameters

Biological Background: TIM23 (TIMM23) Function and Localization

• Encoded by the TIMM23 gene, Tim23 is a multi-pass membrane protein of the mitochondrial inner membrane.
• Essential component of the TIM23 complex, which mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Related references: PMID:10339406
• Protects PINK1 from OMA1-mediated degradation, facilitating PINK1 accumulation at the outer mitochondrial membrane during mitophagy. Related references: PMID:37160114
• Localizes exclusively to the mitochondrion inner membrane.
• Features transmembrane helices and functions in protein transport and translocation.
• Molecular weight is approximately 22 kDa (calculated 21943 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 7–72 of human TIMM23 (NP_006318.1), targeting the N-terminal region; consider Western blot detection at ~22 kDa.
• For Western blot (WB) and immunoprecipitation (IP), validate the antibody in your specific cell or tissue lysate, as cross-reactivity is reported for human, mouse, and rat.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), anticipate mitochondrial staining patterns; include appropriate organelle markers for co-localization.
• For ELISA and other quantitative applications, perform titration experiments to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, technical services, and consulting, supporting manufacturers, labs, and institutes from concept to clinic. For mitochondrial biology and protein import research, we offer validated antibodies like the anti-TIM23 monoclonal antibody, along with complementary reagents and custom sourcing solutions. Whether you need optimized antibody pairs, bulk ancillary reagents, or rare target raw materials, CamelBio streamlines your procurement with quality and reliability.