Anti-TIM-3/HAVCR2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8TDQ0

Core Product Specifications and Parameters

Biological Background: TIM-3/HAVCR2 Function and Localization

• TIM-3 (Hepatitis A virus cellular receptor 2) is a type I transmembrane protein belonging to the immunoglobulin superfamily and TIM family, also known as T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3) and CD366.
• Functions primarily as an inhibitory immune checkpoint receptor on T-cells, attenuating TCR signaling and promoting T-cell exhaustion; it inhibits Th1-mediated auto- and alloimmune responses, contributing to immunological tolerance (PMID:14556005).
  Related references: PMID:14556005
• Acts as a receptor for galectin-9 (LGALS9), which induces apoptosis of T-cells and suppresses effector functions, though this interaction has been debated (PMID:16286920, PMID:24337741, PMID:23555261).
  Related references: PMID:16286920 PMID:24337741 PMID:23555261
• Also binds phosphatidylserine (PtSer) in a calcium-dependent manner, mediating phagocytosis of apoptotic cells and regulating innate immunity, particularly in dendritic cells and macrophages (PMID:24825777).
  Related references: PMID:24825777
• Expressed in various immune cell subsets: Th1 lymphocytes, regulatory T cells (Tregs), dendritic cells, natural killer (NK) cells, and epithelial tissues; expression is upregulated in chronic viral infections such as HCV and HIV, contributing to immune exhaustion.
• Plays dual roles in innate immunity: on NK cells, it can enhance IFN-gamma production in response to LGALS9 (PMID:22323453) or suppress NK cell-mediated cytotoxicity (PMID:22383801).
  Related references: PMID:22323453 PMID:22383801
• Undergoes post-translational modifications including glycosylation, palmitoylation, and phosphorylation; features disulfide bonds and an Ig-like V-type domain essential for ligand binding.
• Subcellular localization: predominantly on the cell membrane and at cell junctions, where it participates in immune synapse formation and signaling.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to the extracellular domain (aa22-202), which is suitable for detecting native and denatured TIM-3 in WB, IHC-P, and ELISA.
• For WB analysis, consider using reducing conditions and whole-cell lysates from Jurkat or activated primary T-cells to confirm the expected band at approximately 33 kDa, though glycosylation may cause a higher apparent molecular weight.
• For IHC-P, antigen retrieval methods (e.g., citrate buffer, pH 6.0) may be required; validate the staining pattern in human tonsil or lymph node tissues, with appropriate negative controls.
• For ELISA, the antibody can be examined as either capture or detection reagent; perform checkerboard titrations with coated recombinant TIM-3 and known positive samples.
• Given the reported cross-reactivity with mouse and rat, consider verifying sequence homology of the immunogen region across species, and validate the antibody in relevant rodent models (e.g., mouse spleen lysates, rat tissue sections).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-TIM-3/HAVCR2 polyclonal antibody is a key tool for immune checkpoint research, cancer immunotherapy, and infectious disease studies. Whether you require rare target sourcing, custom antibody development, or scalable production, CamelBio streamlines your procurement journey from concept to clinic.