Anti-TIM-3/HAVCR2 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q8TDQ0

Core Product Specifications and Parameters

Biological Background: TIM-3/HAVCR2 Function and Localization

• HAVCR2/TIM-3 is generally accepted as an inhibitory receptor that regulates innate and adaptive immune responses, with context-dependent activity. It regulates macrophage activation. Related references: PMID:24825777, PMID:11823861
• It inhibits Th1-mediated auto- and alloimmune responses and promotes immunological tolerance. On CD8+ T-cells, it attenuates TCR signaling by blocking NF-kappaB and NFAT, reducing IL-2 secretion, possibly via association with LCK. Related references: PMID:14556005, PMID:24337741, PMID:26492563
• TIM-3 is a receptor for LGALS9, leading to suppression of T-cell responses and apoptosis of antigen-specific cells, though this role has been challenged. Related references: PMID:16286920, PMID:24337741, PMID:23555261
• It also functions as a receptor for phosphatidylserine, mediating phagocytosis of apoptotic cells and positively regulating innate immunity in dendritic cells.
• On natural killer (NK) cells, TIM-3 acts as a coreceptor enhancing IFN-gamma production in response to LGALS9, but can also suppress NK cell-mediated cytotoxicity. Related references: PMID:22323453, PMID:22383801
• Expressed in Th1, Treg cells, dendritic cells, NK cells, and epithelial tissues; localized at the cell membrane and cell junctions.
• TIM-3 is a transmembrane glycoprotein with immunoglobulin-like domains, disulfide bonds, and palmitoylation, with a predicted molecular weight of ~33 kDa.

Experimental Guidance and Technical Tips

• The antibody was generated using a recombinant protein immunogen corresponding to human TIM-3. For applications such as WB, IF-P, IHC-P, and ELISA, consider optimizing antibody concentrations and validating specificity in the relevant sample system.
• In WB experiments, a band of approximately 33 kDa is expected based on the predicted molecular weight. Adjust blocking and detection conditions as needed for optimal signal-to-noise ratios.
• For cross-reactivity with mouse, verify by sequence homology and test under appropriate experimental conditions, as reactivity may vary between sample types.

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CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. This TIM-3/HAVCR2 antibody supports immuno-oncology and immune checkpoint research, enabling diagnostic manufacturers and labs to secure reliable reagents for assay development. Let our B2B supply network streamline your workflow from concept to clinic.