Anti-TIM-1/KIM-1/HAVCR Rabbit Polyclonal Antibody - Q5QNS5

Core Product Specifications and Parameters

Biological Background: TIM-1/KIM-1/HAVCR Function and Localization

• TIM-1/KIM-1/HAVCR, encoded by the Havcr1 gene, is a type I transmembrane glycoprotein also known as Kidney injury molecule 1 (KIM-1) and T cell immunoglobulin and mucin domain-containing protein 1 (TIMD-1). It belongs to the TIM family of phosphatidylserine receptors.
• Acts as a phosphatidylserine receptor, playing a critical role in regulatory B-cell homeostasis, including generation, expansion, and suppressor functions.
  Related references: PMID:21821911 PMID:25645598 PMID:32668241
• Functions in efferocytosis and phagocytosis: binds phosphatidylserine on apoptotic cells, facilitating their engulfment by kidney epithelial cells and also enabling phagocytosis of bacteria.
• Serves as a ligand for P-selectin/SELPLG, regulating activated T-cell trafficking during inflammatory responses, thereby controlling T-cell accumulation in the central nervous system and autoimmune disease induction.
  Related references: PMID:24703780
• Regulates T-cell proliferation and expression of anti-inflammatory cytokines and co-inhibitory ligands, including IL-10.
  Related references: PMID:15793576 PMID:25582854 PMID:25645598
• Subcellular localization includes the cell membrane, phagocytic cup, and cytoplasmic vesicles/phagosomes, consistent with its role in phagocytosis and signaling.
• Post-translational modifications include N-linked glycosylation and disulfide bonds, typical of immunoglobulin superfamily members, which may influence ligand binding and stability.
• Tissue-specific expression: expressed by stimulated T-cells and at higher levels on B-cells, both constitutively and after activation, with upregulation during primary antigen stimulation.
  Related references: PMID:11725301 PMID:21821911

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22-237), which is likely recognized by the antibody; thus, it may detect both full-length and soluble ectodomain forms. Consider optimizing antigen retrieval or denaturing conditions for Western blot to detect the 33 kDa band.
• For ELISA, use indirect or sandwich ELISA with appropriate coating buffers, and validate specificity using recombinant TIM-1 protein or cell lysates from mouse T-cell or kidney epithelial cell lines.
• Cross-reactivity with human TIM-1 is reported; however, due to polyclonal nature, confirm cross-reactivity in your specific sample system and consider batch-to-batch variability.
• When studying tissue-specific expression, note that TIM-1 is upregulated upon injury or activation; therefore, include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio connects diagnostic manufacturers and research laboratories with high-quality antibodies like this anti-TIM-1/KIM-1/HAVCR polyclonal antibody, supporting studies in immunoregulation and kidney disease. Beyond single reagents, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, streamlining your project from concept to clinic.