Anti-TIGIT Rabbit Polyclonal Antibody for WB, ELISA - Q495A1

Core Product Specifications and Parameters

Biological Background: TIGIT Function and Localization

• TIGIT is an inhibitory receptor that suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Related references: PMID:19011627
• It binds to ligands PVR/CD155 or NECTIN2/CD112 on antigen-presenting cells, sending inhibitory signals through Src family kinases. Related references: PMID:23154388
• TIGIT associates with beta-arrestin-2/ARRB2 to recruit SHIP1, suppressing TRAF6 autoubiquitination and inhibiting NF-kappa-B signaling. Related references: PMID:24817116
• Also acts as a receptor for NECTIN4 to inhibit NK cell cytotoxicity. Related references: PMID:32503945
• Subcellular localization: Cell membrane.
• Tissue specificity: Expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells, upregulated upon activation.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 167-244) lies within the cytoplasmic domain; consider this region suitable for total TIGIT detection in denaturing Western blots and verify epitope accessibility in native assays.
• Predicted molecular weight is ~26 kDa, but glycosylation may cause higher apparent bands; validate with positive controls like activated T-cells or NK cells.
• For ELISA, perform titration and specificity checks using recombinant or native TIGIT; cross-reactivity with mouse TIGIT should be verified in your system.
• TIGIT upregulation upon immune activation suggests using stimulated cells or relevant tissues; further optimization is needed for applications beyond WB and ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-TIGIT antibody supports immuno-oncology research and assay development; our supply chain includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with CamelBio to streamline your transition from concept to clinic.