Anti-TIGD1/Eeyore Rabbit Polyclonal Antibody for WB and ELISA - Q96MW7

Core Product Specifications and Parameters

Biological Background: TIGD1 Function and Localization

• TIGD1 (Tigger transposable element-derived protein 1) is a human protein that belongs to the Tigger family of DNA transposon-derived proteins.
• It is also known by the alias EEYORE.
• Encoded by the TIGD1 gene (Gene ID: 200765).
• Subcellular localization is predominantly nuclear, consistent with its DNA-binding role.
• Classified under the keyword "DNA-binding", suggesting the presence of domains that interact with nucleic acids.
• Identified in high-throughput proteomics studies and is part of the human reference proteome.
• The canonical protein has a calculated molecular weight of 67,299 Da (approximately 67 kDa).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 1–300, covering a significant portion of the N-terminal region. This may generate polyclonal antibodies recognizing multiple epitopes.
• For Western blotting, consider validating the antibody using nuclear extracts from human, mouse, or rat cell lines, given the reported cross-reactivity.
• In ELISA applications, the recombinant immunogen can serve as a positive control; ensure to include appropriate negative controls to assess background.
• Due to the nuclear localization of TIGD1, subcellular fractionation may improve signal-to-noise ratio in WB assays.
• Because this is a polyclonal antibody, lot-to-lot consistency should be verified, and blocking conditions may need optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with streamlined access to a comprehensive range of IVD raw materials. For TIGD1/Eeyore research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—all backed by our expert technical and consulting support. Partner with CamelBio to accelerate your project from concept to clinic with reliable, one-stop procurement solutions.