Anti-TIGAR Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NQ88

Core Product Specifications and Parameters

Biological Background: TIGAR Function and Localization

• TIGAR (TP53-induced glycolysis and apoptosis regulator), also known as FR2BP or C12orf5, functions as a fructose-2,6-bisphosphatase that hydrolyzes fructose-2,6-bisphosphate and fructose-1,6-bisphosphate. Related references: PMID:19015259

• It acts as a negative regulator of glycolysis by reducing intracellular fructose-2,6-bisphosphate levels in a p53/TP53-dependent manner, thereby activating the pentose phosphate pathway (PPP) and increasing NADPH production. Related references: PMID:16839880 PMID:22887998

• By boosting NADPH levels, TIGAR promotes reduced glutathione generation, which decreases reactive oxygen species (ROS) and protects cells from oxidative stress- and metabolic stress-induced apoptosis. Related references: PMID:16839880 PMID:19713938 PMID:22887998

• The protein localizes to the cytoplasm, nucleus, and mitochondrion, consistent with its roles in metabolic regulation and apoptosis.

• In cancer cells, TIGAR supports survival under hypoxia by inhibiting glycolysis and shifting metabolism toward mitochondrial respiration; it also facilitates DNA repair through PPP activation during genome stress. Related references: PMID:22887998 PMID:25928429 PMID:23726973

• TIGAR is expressed in brain, breast tumors, and glioblastomas, highlighting its relevance in both normal tissues and oncological contexts. Related references: PMID:22887998 PMID:21820150

• Post-translational modifications include acetylation, and the protein is implicated in autophagy and apoptotic pathways.

Experimental Guidance and Technical Tips

• For Western blot analysis, confirm the detection of a band at approximately 30 kDa, matching the predicted molecular weight of TIGAR. Verify specificity by pre-incubating the antibody with the immunizing peptide if non-specific bands appear.
• In immunofluorescence (IF/ICC), optimize fixation and permeabilization protocols to visualize TIGAR in its expected cytoplasmic, nuclear, or mitochondrial compartments depending on cell type and stress conditions.
• The antibody cross-reacts with mouse and rat samples; include appropriate species-specific positive and negative controls when working across these species.
• For ELISA applications, titrate the antibody to determine optimal coating and detection concentrations, and validate using recombinant TIGAR protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting your TIGAR research in cancer biology, apoptosis, and metabolic stress. We offer validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents, along with customized sourcing for rare targets. Whatever your diagnostic development needs, CamelBio streamlines procurement from concept to clinic.