Anti-TIFY10A Polyclonal Antibody for WB, ELISA - Q9LMA8

Core Product Specifications and Parameters

Biological Background: TIFY10A Function and Localization

• Protein TIFY10A (also known as Jasmonate ZIM domain-containing protein 1 or JAZ1) is a nuclear and membrane-localized repressor of jasmonate signaling.
• It functions as a key component of jasmonate perception; jasmonoyl-isoleucine (JA-Ile) promotes interaction between COI1 and TIFY10A, forming a high-affinity co-receptor complex with inositol pentakisphosphate.
• TIFY10A is a member of the TIFY protein family involved in plant defense and developmental processes.
• Strongly expressed in root tips, indicating a role in root growth regulation in response to jasmonates.
• Post-translational modifications include acetylation and ubiquitination (Ubl conjugation), which may regulate its stability and activity.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.
• Also implicated in host-virus interactions, linking jasmonate signaling to viral defense mechanisms.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide from amino acids 154-253 of TIFY10A; performance may vary depending on target accessibility and sample preparation.
• For Western blotting, expect a specific band around 28 kDa in Arabidopsis thaliana protein extracts; validate using positive and negative controls (e.g., jaz1 mutant lines or overexpressors).
• In ELISA, optimize coating concentration and antibody dilution using purified recombinant TIFY10A or peptide-BSA conjugates.
• Consider testing cross-reactivity in closely related species if required for comparative studies.
• Include appropriate blocking and wash conditions to minimize background, especially for membrane-localized protein detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing experience for IVD raw materials, connecting researchers and diagnostic developers with rigorously validated antibodies like this anti-TIFY10A polyclonal antibody to support plant defense and jasmonate signaling research. Beyond individual antibodies, we provide integrated procurement solutions, including ancillary reagents, bulk order flexibility, and custom sourcing for rare or emerging targets—all optimized to accelerate your workflow from concept to clinic.