Anti-TIA1 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P31483

Core Product Specifications and Parameters

Biological Background: TIA1 Function and Localization

• TIA1 (T-cell-restricted intracellular antigen-1) is a cytotoxic granule-associated RNA-binding protein that regulates alternative pre-mRNA splicing and mRNA translation by binding to uridine-rich (U-rich) RNA sequences. Related references: PMID:11106748 PMID:12486009 PMID:17488725
• It modulates alternative splicing of apoptosis-related genes, such as FAS, by promoting inclusion of exon 6 to generate the pro-apoptotic membrane-bound receptor. Related references: PMID:11106748 PMID:17488725 PMID:1934064
• TIA1 is involved in translational repression by binding to AU-rich elements (AREs) in 3' UTRs of target mRNAs, including TNF and PTGS2, and participates in stress granule formation upon cellular stress.
• Alternative splicing produces at least two isoforms: a longer isoform (p40-TIA-1) and a shorter isoform, with the longer isoform displaying enhanced splicing regulatory activity.
• The protein localizes to both the nucleus and cytoplasm, and upon stress, it rapidly translocates to cytoplasmic stress granules where it contributes to translational arrest. Related references: PMID:10613902
• TIA1 is expressed across diverse tissues, including heart, small intestine, kidney, liver, lung, skeletal muscle, testes, pancreas, and ovary.
• Mutations in TIA1 are associated with amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases, underscoring its role in neuronal health.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 287-386 of human TIA1) lies within a region that is highly conserved between human and mouse, supporting the observed cross-reactivity. Confirm homology for other species of interest.
• For Western blotting, the expected band is around 43 kDa, but multiple bands may appear due to alternative splicing isoforms or post-translational modifications. Validate in the relevant sample system.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), TIA1 can be detected in both nuclear and cytoplasmic pools. To visualize stress granules, consider treating cells with stressors (e.g., sodium arsenite) prior to fixation.
• Optimal dilutions and experimental conditions should be determined empirically for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-TIA1 polyclonal antibody supports research into RNA processing, stress biology, and apoptosis. For bulk orders or customized sourcing of TIA1-related reagents and ancillary materials, our team ensures reliable supply and technical support.