Anti-Thyroid Stimulating Hormone (TSH) Monoclonal Antibody for IF, IHC-P, ELISA - P01215/P01222

Core Product Specifications and Parameters

Biological Background: Thyroid Stimulating Hormone Function and Localization

• The glycoprotein hormones alpha chain (CGA) serves as the common alpha subunit for the heterodimeric hormones Thyroid Stimulating Hormone (TSH), Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH), and Chorionic Gonadotropin (CG).
• TSH is composed of the alpha chain encoded by CGA and a hormone-specific beta chain (TSHB), which together bind to the TSH receptor on thyroid cells to regulate thyroid hormone production.
• The CGA protein is a secreted glycoprotein with a molecular weight of approximately 13 kDa, while the TSHB beta chain is about 16 kDa; both are essential for functional hormone assembly.
• Subcellular localization: The alpha chain is processed through the secretory pathway and secreted into the bloodstream, where it circulates as part of the active hormone complex.
• Key post-translational modifications: The CGA subunit contains multiple disulfide bonds and N-linked glycosylation sites, which are critical for proper folding, stability, and receptor interaction.
• UniProt keywords include: 3D-structure, Direct protein sequencing, Disulfide bond, Glycoprotein, Hormone, Proteomics identification, Reference proteome, Secreted, and Signal.

Experimental Guidance and Technical Tips

• Based on the immunogen design (recombinant fragments of CGA and TSHB), this antibody may recognize both the alpha chain and the TSH beta chain. Validate specificity in your assay system using appropriate controls.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval using heat-induced epitope retrieval (HIER) in citrate or EDTA buffer may improve staining. Optimize retrieval conditions for your samples.
• In immunofluorescence (IF-P), ensure adequate permeabilization (e.g., 0.1% Triton X-100) and consider co-staining with organelle markers to confirm the expected secretion pattern.
• When used in ELISA, the antibody can potentially serve as a capture or detection reagent. Titrate antibody concentration and validate compatibility with your sample matrix and detection system.
• Because TSH shares the alpha chain with other glycoprotein hormones (LH, FSH, CG), cross-reactivity with these hormones should be assessed if the assay context demands high specificity for TSH alone.

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